To meet the requirements of a sustainable agricultural economy such as quality, resource conservation, and efficiency, the interaction between the cultivation parameters of plants and the resulting plant ingredients are becoming more and more interesting, especially regarding secondary metabolites. Therefore, the cultivation of lovage under the influence of light-emitting diode illumination and controlled environment conditions was studied and data on the rutin concentrations obtained are presented. Different extraction agents and processes were tested for the treatment of the leaves and the rutin was analyzed using high performance liquid chromatography (HPLC) and ultraviolet-visible spectroscopy (UV-Vis spectroscopy). UV-Vis spectroscopy was found to provide a simple and rapid method of predicting the rutin concentration. The significant parameter regarding the rutin concentration obtained was light intensity and overall rutin concentrations in the range of 17,005 mg to 34,759 mg of rutin per kg dry leaves were found.