Urbanization and the limited environmental carrying capacity of our planet are crucial challenges for human kind. Industrial value creation and the production of goods have a significant role in the worldwide emissions pattern. A biologically oriented conception of technical systems with the goal of a sustainable added value is summarized as “biological transformation”. By bringing together the concentration of consumption, waste generation and more, in urban areas, with the biologicalisation of industrial value creation an opportunity arises to actively create bio-based positive urban production. Based on the principles of the biological transformation a framework is derived, stretching the design space between spatial urban levels and production life cycle stages, thereby fostering positive bio-based urban production. The design space is then applied, allocating current realization and envisioned cases as well as giving recommendations for future research topics.